Fiber digestion, VFA production, and microbial population changes during in vitro ruminal fermentations of mixed rations by monensin-adapted and unadapted microbes

نویسنده

  • P. J. Weimer
چکیده

Abbreviations: aNDF, neutral detergent fiber after -amylase treatment; A:P, molar ratio of acetate to propionate; CP, crude protein; DM, dry matter; HPLC, high-performance liquid chromatography; iBut, isobutyrate; iVal, isovalerate; IVaNDFD, in vitro aNDF digestibility; Mol frac, molar fraction; NFC, non-fiber carbohydrate; qPCR, quantitative polymerase chain reaction; RPS, relative population size; rRNA, ribosomal ribonucleic acid; TMR, total mixed ration; VFA, volatile fatty acids; 2MB, 2-methylbutyrate. ∗ Corresponding author. Tel.: +1 608 890 0075; fax: +1 608 890 0076. E-mail addresses: [email protected], [email protected] (P.J. Weimer), [email protected] (D.M. Stevenson), [email protected] (D.R. Mertens), [email protected] (M.B. Hall). 1 Present address: Mertens Innovation and Research LLS, 6427 County Road A, Belleville, WI 53508-9727, USA. Tel.: +1 608 424 6848. 0377-8401/$ – see front matter. Published by Elsevier B.V. doi:10.1016/j.anifeedsci.2011.06.002 P.J. Weimer et al. / Animal Feed Science and Technology 169 (2011) 68– 78 69

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

The Effects of Various Essential Oils of Medical Plant Seeds and Spices on Digestion Characteristics and Population Changes of Ruminal Anaerobic Fungi in in vitro Condition

The effect of essential oils (EO) of medical plant seeds and spices on rumen microbial fermentation of alfalfa hay, sugar beet pulp and barley grain (as substrate) were evaluated under in vitro conditions. In vitro incubations were carried out using the gas production method with glass syringes. Treatments were as follows; a control (no additive), monensin, EO of cinnamon, black pepper seed, cu...

متن کامل

In vitro assessment of the digestibility of forage based sheep diet, supplemented with raw garlic, garlic oil and monensin

The effect of inclusion of garlic essential oil (EO) at 33, 66 and 100 μg mL-1, raw garlic (GAR) at 5, 10 and 15 mg mL-1 and monensin (MON) at 7.5 μg mL-1 of incubation medium on organic matter digestibility (OMD) was studied with in vitro gas production, ANKOM daisyII and conventional in vitro (IVOMD) methods. The material was incubated with sheep ruminal fluid and the experimental design was ...

متن کامل

High Levels of Monensin to Mid Lactating Dairy Cows: Nutrient Digestibility, Ruminal Fermentation and Microbial Protein Synthesis

The aim of this study was to evaluate the nutrient digestibility, ruminal fermentation and microbial protein synthesis of mid-lactating cows fed high dietary levels of monensin. Twelve Holstein cows were distributed into four 3 × 3 latin squares and assigned to the following treatments: control (CON), monensin 24 (M24, addition of 24 mg monensin/kg diet DM) and monensin 48 (M48, addition of 48 ...

متن کامل

Effects of the antibiotic monensin and an inhibitor of methanogenesis on in vitro continuous rumen fermentations.

1. The effects of a methane inhibitor, ICI 111075, and a propionate enhancer, monensin, were studied using in vitro continuous fermenters. 2. Both compounds increased the yield of substrate energy, carbon and hydrogen in volatile fatty acids (VFA). This was mainly due to an increase in the molar proportion of propionic acid. 3. Improved yields of VFA were accompanied by reductions in methane pr...

متن کامل

Effects of organic acid and monensin treatment on in vitro mixed ruminal microorganism fermentation of cracked corn.

The objective of this study was to determine the effects of organic acids and monensin on the in vitro fermentation of cracked corn by mixed ruminal microorganisms. Ruminal fluid was collected from a steer fed 36.3 kg of wheat silage and 4.5 kg of concentrate supplement once daily. Mixed ruminal microorganisms were incubated in anaerobic media that contained 20% (vol/vol) ruminal fluid and .4 g...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره   شماره 

صفحات  -

تاریخ انتشار 2011